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2012 Meeting of the North Carolina Branch of the American Society for Microbiology

Sponsors & Vendors :




The American Society
for Microbiology


NC State University
Department of Microbiology




Association of
Southeastern Biologists




Nikon Instruments



Life Technologies


Sigma Xi

Sigma Xi





Advanced Microscopy Group


NC Academy of Science


When :

Saturday, October 6th, 2012

Where :

The Sigma Xi Center
3106 East NC Highway 54
Research Triangle Park, NC 27709

Parking is available at the Center, no permit is required

Sigma Xi Center

Accommodations :

For those coming from outside the area, there is a wide range of accommodations available in the immediate area in all price ranges.The closest hotels are clustered around I-40 the Highway 55 exit (278) just to the West of the Sigma Xi center, and the Miami Blvd exit (281) just to the East of the Sigma Xi center.

The very closest hotel is the Radisson Hotel RTP, just a couple of hundred yards away.

Registration and abstract submission :

Online pre-registration for the meeting is now closed, nd the deadlne for abstract submission has passed. However, you can still register and pay the regiustration fee ($20) at the door.

If you have pre-registered, please verify your registration info - email Jim Brown with any corrections or other problems.

Payment :

The meeting registration fee is $10. If you joined or renewed your NC branch membership when you renewed your ASM membership this year, then this $10 is the only cost for the meeting. Registrants will receive a program (including abstracts and attendee roster), a box lunch and morning and afternoon refreshments.

If you are not an NC ASM member for 2012, there is an additional $10 branch membership fee (this is the same fee the ASM charges), for a total of $20.

The Registration and/or membership fee is payable at the door; online pre-payment is now closed.

Information for presentors :

Short talks

We are planning for ca. 15 minutes for each talk. This includes Q&A and switching time, so plan on about 12 minutes for your talk, a couple of minutes for Q&A, leaving us 1 minute for switching to the next speaker.

Plan on bringing your presentation on a USB memory stick. If this isn't possible, let us know ASAP so we can arrange some other method to transfer your presentation.

The computer we'll be using will be a PC with Microsoft Powerpoint and Adobe Acrobat Reader. This computer MAY NOT HAVE THE VERY LATEST VERSION OF POWERPOINT! In order to ensure you can run your presentation hassle-free, be sure to save it in "compatibility mode" - in other words, in PPT format rather than PPTX format. We'll be able to translate your files if need be, but there is some danger to translational issues if we have to do this.

If you create your presentation on a Mac, you should be OK, but please test it out on a PC in advance. Some graphics types aren't always handled well on PCs; Postscript, PDF and TIFF.

If you have any questions or concerns about your presentation, please feel free to contact me at


Posters will be mounted using small paper clamps onto 36" x 48" hardboard sheets, which can be placed onto the easels either horizontally (landscape) or vertically (portrait). We will provide clamps, boards, and easels; all you need to bring is your poster. If your poster is in panels or pieces, you should let me ( know RIGHT AWAY so I can get styrofoam backing sheets so that you can pin your poster panels into place.

Schedule :


Poster and talk set-up
Coffee break
Award Committees meeting/organization

9:00 Amy Grunden Welcome & Introductory comments
Session 1: Jim Brown, Chair
9:15 Floyd Inman III Liquid Media Development for the Mass Production of the Entomoparasitic Nematode Heterorhabditis bacteriophora
9:30 Rinu Kooliyottil Critical Aspects in the Mass Production of Beneficial Nematodes
9:45 Len Holmes Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora Utilizing Fermentation Technology

Poster session 1 (Odd numbered posters should be attended)
Coffee break
Vendors & Exhibitors

Session 2: Eric Anderson, Chair

Michael I. Betteken

Oxygen Induced Resistance to Tert-butyl Hydroperoxide is Mediated by Dps in Bacteroides fragilis
11:15 David A. Martinson

The Brucella abortus Irr Protein Directly Regulates the Transcription of Genes Encoding for Iron Uptake Systems, and the Iron Responsive Activity of Irr Is Dependent upon an Internal Heme Binding Motif

11:30 Elhassanny Ahmed FtrABCD is a Ferrous Iron Transporter that Is Required for Virulence in Brucella abortus 2308
11:45 Clayton C. Caswell A Novel Small Regulatory RNA is Regulated by and Potentially Regulates the General Stress Response in Brucella abortus 2308
12 Noon

Unattended Poster Session
Exhibitors & Vendors

Session 3: Wrennie Edwards, Chair
1:00 Maria C. White Equine Herpesvirus Type 1 (EHV-1) Mediated Oncolysis and Viral Cell to Cell Spread in Human Glioblastoma Multiforme Cells Are Enhanced by Valproic Acid
1:15 Syed Sultan Motility but Not the Possession of Periplasmic Flagella is Crucial for the Enzootic Life Cycle of Borrelia burgdorferi
1:30 Yajuan Lin Estimating the Genotype Specific in situ Activity of Prochlorococcus from rRNA Content and Cell Size
NC Invitational Talk: Seán O’Connell, Chair
1:45 Brauer

NC Invited Lecture

Department of Biology
Appalachian State University

Microbial Biogeochemical Cycling of Manganese

I am broadly interested in the biogeochemistry, ecology and diversity of microorganisms in various environments, including rivers, coastal margins, wetlands/estuaries, deep-sea hydrothermal vents, and caves; and I am interested in studying the impact of these microorganisms on the biogeochemical cycling of Fe, Mn and C in the environment.


Poster session 2 (Even numbered posters should be attended)
Coffee break
Vendors & Exhibitors

ASM Branch Lecture: Amy Grunden, Chair
3:15 Freitag

ASM Branch Lecture

Department of Microbiology and Immunology
University of Illinois at Chicago

From soil to cytosol: the pathogenic transition of the environmental
bacterium Listeria monocytogenes

  Dr. Freitag has been continuously funded for her research on different aspects of Listeria monocytogenes pathogenesis for over 15 years. She is internationally recognized for her work on microbe-host interactions, and she has published more than 50 papers and book chapters while also serving as a member of journal editorial boards that include Infection and Immunity and Molecular Microbiology. Dr. Freitag has been a member or ad hoc member on numerous NIH study section panels, and she has served as an ad hoc reviewer for international foundations such as the Wellcome Trust. Dr. Freitag has been an invited speaker at more than 80 conferences, seminars, and colloquia, and has participated in a number of Science Career forums organized by various foundations, community colleges, and universities. She maintains an active interest in science education, and has been actively engaged in high school teacher and student science research mentoring programs, as well as serving for five years as an ASM judge for the Intel International Science and Engineering Fair.
4:15 Amy Grunden

Concluding remarks

4:30 Jim Brown

Business meeting
Officer elections


Poster presentations


Anthony D. Angotti

Vibriobactin Mediated Biofilm Formation in Vibrio cholerae


Matt K. Bowen

Microbial Kinetics of Glucose Utilization in Photorhabdus luminescens


Steven R. Cockerell

Norspermidine Responsive Biofilm Development in Vibrio cholerae


Bridget E. Conley

Generation of Campylobacter rectus CiaB mutants


Katy M. Davis

Predictable Cycles and Pulse Disturbances: What Drives Microbial Abundances in a Shallow North Carolina Estuary?


Maria de Oca

Temperature and Nutrient Concentrations Determine Prochlorococcus Genetic Diversity in the Surface Pacific Ocean


Kristen N. Delaney

Campylobacter jejuni Gene Cj0372 (Ortholog of E. coli gspS), is Temperature Regulated and Plays a Role in Cell Size and Resistance to Oxidative Stress


Amy A. Devlin

Characterization of novel, secreted Borrelia burgdorferi Type I IFN-stimulatory ligand(s)


Robert. M. Dodder

Iron Uptake as a Target for Antimicrobial Development in Pseudomonas aeruginosa


Keely Dulmage

Uncovering the Regulatory Relationship between Archaeal Chromosomes and Histone Proteins


Dylan Fehl

The Use of Natural Products to Augment the Oncolytic Activity of Vesicular Stomatitis Virus


Rabin Gyawali

Survival and Changes in Cell Morphology of Escherichia coli O157:H7 in the Presence of Caffeine


Haylea Hannah

The Role of Polysaccharide Degrading Enzymes in the Interaction of Agrobacterium tumefaciens with Plants


Ashley N. Hawkins

Methanogenesis in Acidic Bogs of the Southern Appalachian Region of North Carolina and Investigation of a Surprising Abundance of Crenarchaeota


Saeed A. Hayek

Use of Sweet Potato (Ipomoea batatas) to Develop a Medium for Cultivation of Lactic Acid Bacteria


Salam A. Ibrahim

Development of Food Protection and Defense Educational Materials and Workshops for Middle/High School and Undergraduate Students


Rushyannah Killens–Cade

Characterization of a recombinant Metallosphaera sedula lipase for use in algal-based biofuel production


Priya Jayaraman

The Role(s) of RNA Sensing TLRs and Adapter Molecules in the Host Defense to Borrelia burgdorferi and in B. burgdorferi RNA-mediated Type I IFN Induction


Rebecca L. Kitchener

Improved Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents


Alyse Larkin

Influence of Ocean Temperature on Prochlorococcus Genetic Diversity Gradients in the North Pacific


Alicia Li

Calcineurin Governs Dimorphism and Virulence of Mucor circinelloides


Akarsh Manne

Study of BB0367, an Rrp1-regulon, and its effect on the enzootic life cycle of Borrelia burgdorferi


Stephanie L. Mathews

The Retention of Pathogenic E. coli O157 by Salad Vegetables


Ki Hwan Moon

Cyclic-adenosine Monophosphate (cAMP): a Potential Second Messenger in Borrelia burgdorferi


Tia Morgan

MtrR Regulates Two Major Lytic Transglycosylases Responsible for Peptidoglycan-Derived Cytotoxin Release and Autolysis in Neisseria gonorrhoeae


Ivan Ndamukong

The Alternative Sigma Factor and its Repressor, EcfO/Reo, Have a Defined Regulon and Protect Bacteroides fragilis Against Prolonged Oxidative Stress


Elizabeth A. Novak

Borrelia burgdorferi Requires Cyclic-di-GMP to Switch from Glucose to Glycerol Metabolism for Survival in the Tick Phase of the Enzootic Cycle


Chirayu Patel

Response of Cervical Cancer Cells to Infection with Vesicular Stomatitis VIrus


Steven Schreck

Characterization of a Halophilic Lipase from Chromohalobacter salexigens for Use in an Algal-based Biofuel System


Horia Todor

The Dynamic Regulation Of Metabolism In Response Nutrients In a Halophilic Archaeon


Christopher S. Ward

DNA extraction method influences apparent diversity of marine bacterioplankton community


Aaron Yerke

Hunting for the collar protein seen in Borrelia burgdorferi periplasmic flagella


Cheuk Man Yung

Seasonal partitioning of Vibrio populations in coastal seawater


Bryan Zorn

Mn(II)-oxidizing fungal populations in caves of the southern Appalachian region

Elections :

The meeting will be concluded by a brief business meeting, including the election of new officers.

If you would like to nominate someone or volunteer to run for office, please feel free to do so by email to Jim Brown.

  • President : Amy Grunden, NCSU (elected last year)
  • President-elect :
    • nominee - Eric Anderson, ECU
  • Secretary : Jim Brown, NCSU (has agreed to continue this year)
  • Treasurer : Wrennie Edwards, Novartis (has agreed to continue this year)
  • Councilor : Ece Karatan, ASU (has agreed to continue this year)
  • Altenrate Councilor : Ed Swords, WFU (has agreed to continue this year)

Presentation Awards :

Web Links :

Printed material :

Contact :

Amy Grunden
4550A Thomas Hall
Campus Box 7615
North Carolina State University
Raleigh, NC 27695
919.513.4295 (voice)
919.515.7867 (fax)

Organizing Committee :

Last updated by James W. Brown | Department of Biological Sciences | College of Sciences | NC State University